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In summary, photodiodes act as current sources similar to photoconductive devices but without photoconductive gain. Gain is often provided by an operational ampli er, which also converts the photocurrent into a voltage proportional to the photon ux. Photodiode geometry and circuits are extended in many ways for functional photodetectors. For example, the p i n diode adds an intrinsic absorption layer between the p- and n-type materials. The intrinsic layer affords a uniform depletion region with a constant acceleration eld. Avalanche photodiodes are strongly reverse biased such that photogenerated charge induces a cascade of electron hole pair ionizations, amplifying the current response by factors of 1000 or more. Finally, Schottky barrier photodiodes rely on the contact potential between a metal and an insulator to produce the space charge region.

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lysins interact with those components of the cell wall that are necessary for bacterial viability (Borysowski et al 2006; Fischetti 2008) For instance, the receptor for S pneumoniae-speci c lysins is choline, an essential component of the pneumococcal cell wall In fact, in none of the hitherto conducted studies have any lysin-resistant bacteria been identi ed (Loef er et al 2001) In two separate studies, both S pneumoniae and B cereus failed to develop resistance to lysin even following repeated exposure to low doses of enzyme (the same results were obtained in experiments carried out on solid media and in liquid cultures; Loef er et al 2001; Schuch et al 2002) Furthermore, B cereus remained sensitive to lysin even after exposure to mutagens that rapidly induced mutations resulting in resistance to novobiocin and streptomycin (Schuch et al 2002).

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On the other hand, it was found that bacteria can be less susceptible to lysins during the stationary phase, probably owing to some changes in the cell wall composition (Borysowski et al 2006) While no cases of bacterial resistance to lysins have been reported as yet, four mechanisms inducing resistance to lysostaphin have been identi ed (Shaw et al 2005; Gr ndling et al 2006; Kusuma et al 2007) These are described in more detail in 7 Interestingly, in some cases the development of resistance to lysostaphin in MRSA results in an increase in susceptibility to methicillin and other antibiotics as well as a reduction in bacterial tness and virulence (Kusuma et al 2007) Moreover, two general mechanisms mediating bacterial resistance to lysozyme have been reported.

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The rst is based on the modi cations of some peptidoglycan components (either O-acetylation of N-acetylmuramic acid residues by O-acetyltransferase or deacetylation of N-acetylglucosamine residues by N-acetylglucosamine deacetylase; Vollmer and Tomasz 2000; Bera et al 2005) Such modi ed residues restrict the access of lysozyme to its substrate The other mechanism involves the production of a lysozyme inhibitor (Binks et al 2005) 62 Gram-negative Bacteria While in Gram-positive bacteria peptidoglycan is easily accessible to recombinant lytic enzymes from outside the cell, in Gram-negative bacteria it is protected by the outer membrane that is impermeable to macromolecules (Vaara 1992) Therefore, Gram-negative bacteria are essentially resistant to lytic enzymes However, the results of some studies indicate that these bacteria can also be killed by recombinant lytic enzymes.

(3:81)

instead of the classical expression (6.49).

Virtually all 10/100 switches are autosensing, meaning that each port automatically adjusts itself for 10 or 100 mbps operation, depending on the device connected to that port. This is an important feature if you plan to have a mix of 10 and 100 mbps devices on your LAN. Figure 5-8 shows the front and rear views of a 16-port switch.

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